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soybean trypsin inhibitor immobilized agarose  (Thermo Fisher)


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    Structured Review

    Thermo Fisher soybean trypsin inhibitor immobilized agarose
    Soybean Trypsin Inhibitor Immobilized Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agarose+immobilized+trypsin/Trypsin/bio_rxiv__64898__2026__03__12__711446-254-11-16
    Average 99 stars, based on 1 article reviews
    soybean trypsin inhibitor immobilized agarose - by Bioz Stars, 2026-10
    99/100 stars

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    Activity Assay:

    Article Title: Towards a generic prototyping approach for therapeutically-relevant peptides and proteins in a cell-free translation system
    Article Snippet: To calculate IC 50 , the initial velocities of the substrate hydrolysis by trypsin in the presence of different concentrations of peptide were fitted to a nonlinear regression curve using the software package Prism (GraphPad Software) followed by determining the corresponding K i value using a tight-binding equation and a K m value of 12 μM. .. Agarose-immobilized trypsin (treated with L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) to reduce the chymotrypsin activity) was purchased from ThermoFisher (#20230) and equilibrated with digestion buffer (0.1 M NH 4 HCO 3 , pH 8.0), aliquoted and stored at 4 °C as 50% slurry (vol/vol). .. 20 μL of mixed trypsin–resin suspension was transferred into the filter-bottom tube, spun at 5000 × g for 2 min and then mixed with SFTI-RGS peptide in digestion buffer containing 0.1 M NH4HCO3 buffer, pH 8.0 at 1:1 or 1:10 protease to peptide ratios followed by incubation for 3 h at 37 °C using the ThermoMixer (Eppendorf).



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